Antibacterial Activity Test of Sweet Corn (Zea Mays L.) Ethanol Extract on Escherichia Coli and Staphylococcus Epidermidis Bacteria
Uji Aktivitas Antibakteri Ekstrak Etanol Jagung Manis (Zea Mays L.) terhadap Bakteri Escherichia Coli dan Staphylococcus Epidermidis
DOI:
https://doi.org/10.58330/manganite.v2i1.301Keywords:
Antibacterial, Ethanol Extract, Escherichia coli, Staphylococcus epidermidis, Sweet Corn PeelAbstract
Sweet corn fruit peel (Zea mays L.) is one of the plant species belonging to the Poaceae family. The aim of the study was to determine the antibacterial activity and the most effective concentration of sweet corn rind in inhibiting the growth of Escherichia coli and Staphylococcus epidermidis bacteria. Types of experimental research in the form of sample collection, manufacture of simplicia, screening of phytochemicals, characteristics of simplicia, preparation of ethanol extract of sweet corn husks with various concentrations of 10%, 15%, 20%, extract characteristics, rejuvenation of Escherichia coli bacteria with EMBA media and Staphylococcus epidermidis with media MSA, as well as testing the antibacterial activity using MHA media for both bacteria with the disc diffusion method in inhibiting the growth of Escherichia coli and Staphylococcus epidermidis bacteria with 1% Chloramphenicol positive control and DMSO negative control. The results of the ethanol extract of sweet corn rind were able to inhibit the growth of Escherichia coli and Staphylococcus epidermidis bacteria. The antibacterial activity test of the ethanol extract of sweet corn rind on Escherichia coli bacteria showed an average inhibition zone of each concentration, namely 10% (11.18 mm), 15% (12 mm), 20% (13.42 mm). And the Staphylococcus epidermidis bacteria showed an average inhibition zone of each concentration, namely 10% (10.72 mm), 15% (12.37 mm), 20% (14.67 mm). In conclusion, the ethanol extract of corn husks has antibacterial activity against Escherichia coli and Staphylococcus epidermidis bacteria.
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Copyright (c) 2023 Muhammad Andry, Tetty Noverita Khairani, Rida Evalina Tarigan, Nursafwati Nursafwati, Muhammad Amin Nasution, Ika Julianti Tambunan, Muhammad Faizal Fathurrohim, Firman Rezaldi
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